Optimizing Growth Conditions for Digoxin Production inDigitalis lanata Ehrh
  
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DOI:10.15806/j.issn.2311-8571.2016.0010
KeyWord:Digitalis lanata Ehrh., mao hua yang di huang, cardenolides, content optimisation, TCM
                 
AuthorInstitution
Herman A. van Wietmarschenab a.TNO Netherlands Organization for Applied Scientific Research, Microbiology & Systems Biology, P.O. Box 360, 3700 AJ Zeist, the Netherlands; b.Sino-Dutch Centre for Preventive and Personalized Medicine, P.O. Box 360, 3700 AJ Zeist, the Netherlands
HansjÖrg Hagelsc cBoehringer Ingelheim Pharma GmbH & Co.KG, c.Binger Str. 173, 55216 Ingelheim/Rhein, Germany
Ron Petersd d.Business Center Klazienaveen, Gantel 12, 7891 XA Klazienaveen, the Netherlands
Jolanda Heisteke e.Jolanda: Heistek Result, Groenloper 7, 2665 VK, Bleiswijk, the Netherlands
Jan van der Greefabf a.TNO Netherlands Organization for Applied Scientific Research, Microbiology & Systems Biology, P.O. Box 360, 3700 AJ Zeist, the Netherlands. b.Sino-Dutch Centre for Preventive and Personalized Medicine, P.O. Box 360, 3700 AJ Zeist, the Netherlands;f.SU BioMedicine, Utrechtseweg 48, Zeist, the Netherlands
Mei Wangbf b.Sino-Dutch Centre for Preventive and Personalized Medicine, P.O. Box 360, 3700 AJ Zeist, the Netherlands;f.SU BioMedicine, Utrechtseweg 48, Zeist, the Netherlands
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Abstract:
      Objective: Digoxin is a therapeutic cardenolide widely used to treat various heart conditions such as atrial flutter, atrial fibrillation and heart failure in both Western as well as Chinese medicine. Digoxin is extracted from cultivated Digitalis lanata Ehrh. plants, known as Mao Hua Yang Di Huang in Chinese medicine. This manuscript presents two studies that were conducted to optimize the cultivation conditions for digoxin production in the TCM Mao Hua Yan Di Huang in a greenhouse under GAP conditions.Methods: Two experiments were designed in which 4 growth conditions were compared. Levels of digoxin, gitoxin, digitoxin, α-acetyldigoxin, β-acetyl-digoxin were measured using HPLC-UV and compared between the conditions. Results: Normal soil, no CO2 enrichment combined with a cold shock was found to be the optimal condition for producing digoxin in the first experiment. Gitoxin content was significantly lower in plants grown in this condition. Mechanical stress as well as the time of harvesting showed no statistically significant differences in the production of cardenolides. In the second experiment the optimal condition was found to be a combination of cold nights, sun screen, fertilizer use and no milled soil. Conclusion: This study shows that digoxin production can be increased by controlling the growth conditions of D. lanata Ehrh. The effect of cold was important in both experiments for improving digoxin production. Cultivating Chinese herbal medicines in optimized greenhouse conditions might be an economically attractive alternative to regular open air cultivation.
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